The Effect of Mild Hemolysis on Serum Glutamic Oxaloacetic Transaminase Enzyme Activity

Abstract

One of the biggest errors in pre-analytics is hemolysis. Hemolysis is a disruption that occurs in the erythrocyte membrane, resulting in the release of hemoglobin. The wavelength at which the parameters measured spectrophotometrically are determined can overlap with the absorption spectrum of hemoglobin. Materials and Methods: the analyte test will be disturbed depending on the level of hemolysis. A study has been conducted on the effect of mild hemolysis (0.05 - 0.1 g / dl hemoglobin) in serum on SGOT enzyme activity. The population in this study were patients who underwent examination at the Mediko Farma Laboratory. Results: From the results of measuring the activity of the SGOT enzyme in normal serum and in serum with mild hemolysis, the following results were obtained: for the value of SGOT activity in normal serum, the lowest value was 18 IU/L and the highest was 36 IU/L, and the average value was 22.68 IU/L. For the activity of SGOT activity in serum with mild hemolysis, the lowest value was 33 IU/L and the highest was 52 IU/L, and the average value was 39.63 IU/L. Conclusion: The percentage of average increase in SGOT activity in serum with mild hemolysis levels was 77.52%. The Wilcoxon test to determine the effect of hemolysis showed that Asymp. Sig (2-tailed) was 0.001, which means that there is an effect of mild hemolysis levels in serum on SGOT enzyme activity.